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List of best Powerpoint Resume Templates. Now you got everything you need. Well, almost everything. I explained what is visual resume, why is dance therapy research it better than traditional resume, why you should create PowerPoint resume and not video and education research, I gave you instructions on research paper, how to create it and some of the best resumes to essays give you some inspiration. Dance Therapy. What did I forget? Oh yeah, the papers research thing from the tittle. You got everything you need to create your own resume from dance therapy research, scratch. But if you don’t want to design everything you can always use finished templates.
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Essay: Gene transfer techniques – pros and cons. Gene transfer is a technique to stably and efficiently introduce functional genes (that are usually cloned) into the target cells. Genetic transfer is the mechanism by which DNA is therapy research, transferred from a donor to a recipient. Once donor DNA is inside the recipient, crossing over essays, can occur. The result is a recombinant cell that has a genome different from either the donor or the paper recipient. A recombination event must occur after transfer in order that the change in the genome be heritable (passed on to the next generation).The genes are the essays blueprints essential to generate all the proteins in our bodies which eventually perform all the biological functions. Therefore, when a gene is efficiently introduced into a target cell of the host, the protein which is encoded by that gene is produced . Gene transfer technologies developed initially as a research tool for studying the gene expression and its function. A range of techniques and naturally occurring processes are utilized for the gene transfer. Techniques are available for the introduction of DNA into many different cell types in culture, either to study gene function and regulation or to paper, produce large amounts of recombinant protein. Cell cultures have therefore been used on a commercial scale to synthesize products such as antibodies, hormones, growth factors, cytokines and viral coat proteins for immunization.The most important application of this technology is in personal uk vivo gene therapy, i.e. the introduction of DNA into the cells of live animals in order to therapy research paper, treat disease.
The extraordinary potential of altered DNA molecule is to give rise to new life-forms that are better adopted for survival. Change in base sequence of DNA leads to healthy lifestyle prevent diabetes, change in protein causing disease condition which can be corrected by manipulation of gene. Much emphasis has been given to the gene manipulation in cardiovascular diseases, parkinson’s disease, lysosomal disorders, ocular gene therapy and osteoarthritis. Overview of gene-transfer strategies: Gene transfer can be achieved essentially via three routes. The most straightforward is direct DNA transfer, the dance physical introduction of foreign DNA directly into the cell. Bibliography! For example, in cultured cells this can be done by microinjection, whereas for cells in vivo direct transfer is often achieved by bombardment with tiny DNA-coated metal particles. The second route is termed transfection, and research paper this encompasses a number of research, techniques, some chemical and some physical, which can be used to persuade cells to take up DNA from their surroundings.
The third is to package the DNA inside an. animal virus, since viruses have evolved mechanisms to naturally infect cells and introduce their own nucleic acid. Therapy! The transfer of foreign DNA into a cell by this route is statement, termed transduction. The choice of methods of DNA transfer depends upon the target cells in which transformation will be performed. It also depends upon dance therapy paper the objectives of gene manipulation. The transfection may be either stable or transient. Although, choice of DNA transfer method is very important, the other important steps are selection of gene, isolation of gene, preparing recombinant DNA and selection of transformed cells. The regeneration of organism with new characteristics is also equally important. The delivery of genes in vitro can be done by treating the cells with viruses such as retrovirus or adenovirus, calcium phosphate, liposomes, particle bombardment, fine needle naked DNA injection, electroporation or any combination of these methods.
These are the personal statement uk powerful tools for research and have possible applications in therapy research paper gene therapy. FACTORS TO CONSIDER WHEN SELECTING A GENE DELIVERY METHOD. The advancement of new technologies has made gene delivery both more efficient yet more confusing given the formidable array of reagents and methods now available. Determining the layout best gene delivery approach for any particular experiment requires careful consideration of therapy paper, a number of different factors as stated below. 1. Cellular Context or Environment – The context or environment of the bibliography cells (i.e., in dance therapy paper vivo vs. in. vitro vs. ex vivo) to which nucleic acids will be delivered is the first factor to consider when. deciding upon a gene transfer method.
The obstacles and challenges posed by delivery to essay healthy prevent diabetes, in. vitro cell cultures are very different than those posed by in vivo or ex vivo cells, and require. different tools and techniques for dance therapy paper, overcoming them. For example, targeting nucleic acids to. particular cells while avoiding other cells is personal layout, usually a major concern for in vivo gene transfer. applications, sometimes a concern for dance research paper, ex vivo applications, but usually not a concern for in vitro. Another important issue that must be addressed when planning in vivo (but not in vitro) gene. delivery studies is the immunogenicity of the gene transfer system. This is particularly important when considering the use of prevent, certain viral vectors discussed previously, which may be highly immunogenic.
Also related to immunogenicity is the issue of general safety, both for the experimenter and for the subject of any study utilizing transfection. Almost always, the use of viral vectors requires very extensive safety precautions than does the use of synthetic transfection methods. 2. Cell or Tissue Type – The origin of the paper cells or tissue to which nucleic acids will be. transferred is healthy prevent, typically the second parameter considered when selecting a gene delivery. method. For a number of different reasons, the difficulty of cellular nucleic acid delivery varies. from cell type to cell type. Because of this, it is always important to review the literature to. determine if the cell type of interest has previously been utilized for gene transfer experiments, which exact methods have been used, and what delivery efficiencies were achieved, i.e., what. percentages of dance therapy research paper, cells actually transfected or transduced, with each method.
Certain cell types are often described as ‘notoriously difficult to transfect’. These include primary cell cultures of many varieties. Successful gene transfer to thesis, such cells. usually requires a good deal of therapy paper, research before selecting any particular gene delivery method. 3. Delivery Efficiency – For most applications, transfecting or transducing as many cells as.
possible is preferred, especially when it is necessary to detect the presence of rare transgene. products. Thus, it is statement for unemployment, important to determine the level of delivery efficiency required for a given. application before deciding on a particular gene transfer method. In most cases, it is found that.
viral vectors provide the dance therapy paper highest delivery efficiencies. For some commonly used and bibliography easily transfected cell lines, cationic lipids nearly match viral vectors in their gene transfer efficiency, while providing safer and much quicker procedures. 4. Stable vs. Transient Gene Delivery – Gene transfer procedures are frequently categorized. by whether the therapy delivered gene remains separate from the host cell chromosome or whether it is integrated into the host cell chromosome. In the first case, known as transient gene delivery, expression of the transgene typically dissipates after a given period of time – usually within. several days – because the expression vector is either degraded or expelled from the host cell.
In the second case, known as stable gene delivery, expression of the transferred gene is. prolonged, or stable, because the vector is integrated into education papers research, the host cell chromosome. Obviously, different applications require different time periods of transgene expression. Thus, a careful. comparison between different gene delivery methodologies will allow a suitable choice for. generating the desired transient or stable expression. In the case of viral vectors, determining whether a particular virus is suited for stable or transient. transduction is simply a matter of learning the general characteristics of the vector. Dance Therapy Research! In the case.
of chemical or physical/mechanical gene transfer, determining whether a given method is. suitable for transient vs. stable delivery may be as easy as reading sales literature from the. manufacturer or checking primary research literature. 5. Type of sources, Delivered Molecule – Another factor to consider when selecting a gene delivery. method is what type of nucleic acid or other molecule is being transfected or transduced. For. example, a reagent that efficiently delivers plasmids may not efficiently deliver DNA. oligonucleotides.
It is dance therapy research, frequently easiest to simply review the literature to learn what methods have been used successfully, and to contact other researchers who have experience delivering the personal statement molecule of interest. 6. Cytotoxicity – Another issue that comes up frequently when choosing among different gene. delivery methods is the cytotoxicity of the various methods and reagents. Some methods, such. as electroporation and gene guns, can be extremely harsh on cells and often result in dance therapy research the death. of a majority of cells. Also, the DEAE-dextran can be quite harsh on some cell types, especially. when a DMSO or glycerol shock step is used as part of the by burgmen procedure. In some applications, such as when a small number of transfectants are required from a recalcitrant cell type, this is.
not a problem. In the majority of applications however, a high percentage of cell death is paper, simply. not acceptable. The plethora of available cationic lipids and polymer reagents have varying. degrees of toxicity with different cell types, and their concentrations during transfection can be. optimized as needed to minimize cell death. 7. Suspension vs. Adherent cells – Most commonly transfected cells grow – and are. transfected – while attached to some type of essays by burgmen, support, whether that support is the therapy research tissue from. which they are derived, some inert membrane or scaffolding, or the surface of education papers, a tissue culture.
plate. Less common are cells that grow while suspended in medium, such as those derived from. blood and other bodily fluids. Suspension cells are generally more difficult to transfect; therefore, it may be necessary to rely on harsher gene delivery methods, such as. electroporation, gene guns, microinjection, or viral vectors for acceptable transfection levels. Also, some cationic lipids can effectively transfect suspension cells, and should be tried when.
possible due to their simplicity and cost-effectiveness. 8. Expertise – The degree of difficulty in performing the various transfection techniques can. vary significantly, and therefore the expertise and experience of the researcher is dance therapy paper, usually an. important factor to consider. For example, methods like microinjection viral transfection methods are invariably more complex than cationic lipids and sources polymer reagents. Also, of the chemical methods, calcium phosphate co-precipitation can be quite tricky due to its sensitivity to slight changes in therapy research experimental conditions. 9. Thesis Statement For Unemployment! Time – Another important factor to dance therapy research, consider is the time required for gene delivery.
Viral. methods, while highly efficient, can be quite time consuming, typically taking anywhere from two weeks to one month to complete. In contrast, chemical and physical/mechanical methods can usually be completed within a few days, but frequently provide lower transfection efficiencies. Statement! Thus, it is not uncommon to have to weigh the research benefits of faster results and less labor against the benefit of higher efficiency. 10 Cost – Finally, cost can be a major factor when deciding on the best gene delivery. method for a given application. The most expensive methods tend to be the. physical/mechanical methods, such as electroporation and gene guns, due to the specialized.
equipment they require.Viral gene delivery is perhaps the second most expensive method, since it requires the vector reagents, extensive time and labor to complete lengthy vector preparation procedures, as well as proper disposal of viral waste materials. The third most expensive gene delivery methods utilize polymers and cationic lipids, which do not require any specialized equipment or lengthy viral vector preparation steps. Such reagents typically cost in the hundreds of dollars, depending on the number of thesis, transfections to be completed. Finally, the least expensive methods utilize calcium phosphate, which is a cheap and abundant mineral, and naked DNA, which obviously requires no transfection reagent cost, but can be highly inefficient. GENE TRANSFER TECHNIQUES. Transformation is the naturally occurring process of gene transfer which involves absorption of the genetic material by a cell through cell membrane causing the fusion of the foreign DNA with the native DNA resulting in the genetic expression of the received DNA. Transformation involves the uptake of “naked” DNA (DNA not incorporated into therapy, structures such as chromosomes) by sources bibliography, competent bacterial cells. Dance Therapy! Cells are only competent (capable of taking up DNA) at a certain stage of their life cycle, apparently prior to the completion of cell wall synthesis . Transformation is usually a natural method of gene transfer but as a result of technological advancement originated the artificial or induced transformation.
Thus there are two types called as natural transformation and artificial or induced transformation. In natural transformation, the foreign DNA attaches itself to the host cell DNA receptor and ucl mdres with the help of the protein DNA translocase it enters the therapy research host cell. The presence of nucleases restricts the entry of sources bibliography, two strands of the DNA, destroys a single strand thus allowing only one strand to enter the host cell. Any DNA that is not integrated into the chromosome will be degraded. This single stranded DNA mingles with the host genetic material successfully. The artificial or induced method of dance therapy research, transformation is done under laboratory condition which is either a chemical mediated gene transfer or done by electroporation..
Genetic engineers are able to induce competency by putting cells in certain solutions, typically containing calcium salts. At the entry site, endonucleases cut the DNA into fragments of 7,000-10,000 nucleotides, and the double-stranded DNA separates into bibliography, single strands. The single-stranded DNA may recombine with the host’s chromosome once inside the cell. Therapy Research! This recombination replaces the research gene in the host with a variant – albeit homologous – gene. DNA from a closely related genus may be acquired but, in general, DNA is not exchanged between distantly related microbes.
Not all bacteria can become competent. Conjugation is a means of gene transfer in therapy many species of essays, bacteria. Therapy Research! Cell-to-cell contact by essay lifestyle prevent diabetes, a specialized appendage, known as the F-pilus (or sex pilus), allows a copy of an F- plasmid (fertility plasmid) to transfer to therapy, a cell that does not contain the plasmid. On rare occasions an F-plasmid may become integrated in the chromosome of its bacterial host, generating what is known as an Hfr (high frequency of recombination) cell. Such a cell can also direct the synthesis of a sex pilus. As the chromosome of the Hfr cell replicates it may begin to cross the thesis statement for unemployment pilus so that plasmid and chromosomal DNA transfers to the recipient cell. Such DNA may recombine with that of its new host, introducing new gene variants. Plasmids encoding genes for virulence factors antibiotic-resistance are passed throughout populations of bacteria, and between multiple species of bacteria by conjugation. In the laboratory, conjugation can be used to transfer disrupted genes on a self-transmissible plasmid, to develop a mutant strain. A gene of interest from a recipient E. coli strain is cloned into a self-transmissible vector and maintained in a donor strain for dance, genetic manipulation. The deleted gene construct is then transferred by conjugation from the donor strain back into the recipient strain.
Transduction is another method for transferring genes from one bacterium to essay healthy prevent diabetes, another; the transfer is dance paper, mediated by bacteriophages. A bacteriophage infection starts when the virus injects its DNA into a bacterial cell. The bacteriophage DNA may then direct the synthesis of new viral components assembled in ucl mdres the bacterium. Bacteriophage DNA is replicated and then packaged within the phage particles. Early in research the infective cycle the phage encodes an enzyme that degrades the DNA of the host cell. Papers Research! Some of these fragments of bacterial DNA are packaged within the bacteriophage particles, taking the place of phage DNA. As the number of phages increases it breaks open (lyse) the cell. When released from the infected cell, a phage that contains bacterial genes can continue to infect a new bacterial cell, transferring the bacterial genes.
Sometimes genes transferred in this manner become integrated into the genome of their new bacterial host by homologous recombination. Such transduced bacteria are not lysed because they do not contain adequate phage DNA for dance therapy research, viral synthesis and these phages can enter an essays alternate life cycle called lysogeny. In this cycle, all the virus’s DNA becomes integrated into the genome of the host bacterium. Dance Research Paper! The integrated phage, called a prophage, can confer new properties to bibliography, the bacterium. Researchers use transduction as a means of gene transfer as it requires a media like virus for dance therapy paper, transferring genes from one cell to the other. Viruses are thus as a tool to ucl mdres, introduce foreign DNA from the selected species to the target organism. There are two types of transduction called as generalized transduction in paper which any of the bacterial gene is transferred via the ucl mdres bacteriophage to the other bacteria and paper specialized transduction involves transfer of limited or selected set of healthy, genes. Viral vectors for dance therapy paper, gene transfer:
The use of viruses as vectors for transduction, i.e. the introduction of genes into animal cells by exploiting the natural ability of the essays by burgmen virus particle, within which the transgene is dance therapy paper, packaged, to adsorb to the surface of the cell and gain entry is being employed . Due to the efficiency with which viruses can deliver their nucleic acid into cells and ucl mdres thesis the high levels of replication. and gene expression it is paper, possible to achieve, viruses have been used as vectors not only for gene expression in cultured cells but also for gene transfer to living animals. Four classes of viral vector have been developed for use in human gene therapy and essays have reached phase 1 clinical trials. These are the retrovirus, adenovirus, herpesvirus and adenoassociated virus (AAV) vectors . Therapy Research! Before introducing the bibliography individual vector systems,we discuss some general properties of viral transduction vectors. Transgenes may be incorporated into. viral vectors either by addition to the whole genome or by replacing one or more viral genes. This is generally achieved either by ligation (many viruses have been modified to incorporate unique restriction sites) or homologous recombination.
For many applications, it is favourable to use vectors from which all viral coding sequences have been deleted.These amplicons (also described as ‘gutless vectors’) contain just the cis-acting elements required for packaging and genome replication. The advantage of such vectors is dance research paper, their high capacity for foreign DNA and the fact that, since no viral gene products are made, the vector has no intrinsic cytotoxic effects. The choice of vector depends on the particular properties of the virus and the intended host, whether transient or stable expression is required and how much DNA needs to be packaged. For example, icosahedral viruses such as adenoviruses and retroviruses package their genomes into preformed capsids, whose volume defines the maximum amount of foreign DNA that can be accommodated. Conversely, rod-shaped viruses such as the baculoviruses form the capsid around the genome, so there are no such size constraints. Ucl Mdres Thesis! There is no ideal virus for gene transfer ‘ each has its own advantages and disadvantages. In recent years, a number of hybrid viral vectors have been developed incorporating the dance therapy research paper beneficial features of two or more viruses. 1) viral vectors that especially efficient at transducing the intended cell type but not other cell types. 2) retroviruses are particularly proficient at delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 1) cannot deliver genes to terminally differentiated cells. 2) viral vectors may be highly immunogenic.
TRANSFECTION BY CHEMICAL METHODS. One of the methods of thesis statement, gene transfer where the genetic material is deliberately introduced into dance therapy, the animal cell in view of studying various functions of proteins and the gene is transfection. This mode of essays, gene transfer involves creation of pores on the cell membrane enabling the cell to receive the foreign genetic material. When transformation is carried out in eukaryotic cells it is research paper, termed as transfection. 1) Calcium phosphate mediated DNA transfer. The process involves a mixture of ucl mdres, isolated DNA (10-100ug) with solution of. calcium chloride and potassium phosphate. Dance Research! . When the personal statement layout uk two are combined, a fine precipitate of the positively charged calcium and dance research the negatively charged phosphate will form, binding the education research DNA to be transfected on its surface .Cells are then incubated with precipitated DNA either in solution or in tissue culture dish. A fraction of cells will take up the calcium phosphate DNA precipitate by a process not entirely understood but thought to dance, be endocytosis. Transfection efficiencies using calcium phosphate can be quite low, in the range of personal layout, 1-2 % . It can.
be increased if very high purity DNA is therapy paper, used and the precipitate allowed to form slowly. 1. Frequency is very low. 2. Integrated genes undergo substantial modification. 3. Many cells do not like having the solid precipitate adhering to bibliography, them. and the surface of therapy research paper, their culture vessel. 4. Integration with host cell chromosome is ucl mdres thesis, random. 5, Due to above limitations transfection applied to somatic gene therapy. This technique is used for introducing DNA into mammalian cells. This process has been a preferred method of identifying many oncogenes. 2)DNA transfer by DAE-Dextran method:
DNA can be transferred with the help of DAE Dextran also. Dance Therapy! DAE-Dextran may be used in the. transfection medium in which DNA is present. This is a polycationic, high molecular weight substance and is convenient for transient assays. The negatively charged DNA binds to the polycation and the complex is taken up by ucl mdres, the cell via endocytosis.
If DEAE (diethylaminoethyl )-Dextran treatment is coupled with Dimethyl Sulphoxide (DMSO) shock, then upto 80% transformed cell can express the transferred gene. The advantage of this method is that, it is cheap, simple and can be used for transient cells. which cannot survive even short exposure of calcium phosphate. The major advantages of the technique are its relative simplicity and speed, limited expense, and remarkably reproducible interexperimental and intraexperimental transfection efficiency. Disadvantages include inhibition of cell growth and induction of heterogeneous morphological changes in cells. Furthermore, the concentration of serum in the culture medium must be transiently reduced during the transfection. It does not appear to be efficient for therapy research paper, the production of stable transfectants. For cells that grow in by burgmen suspension, electroporation or lipofection is usually preferred, although DEAE-dextran-mediated transfection can be used. Transfer of DNA by polycation-DMSO:
As calcium phosphate method of DNA transfer is reproducible and efficient but there is narrow range of paper, optimum conditions. DNA transfer by. Another polycationic chemical,the detergent Polybrene is carried out to increase the adsorption of education research, DNA to the cell surface followed by a brief treatment with 25-30% DMSO to increase membrane permeability and dance therapy paper enhance uptake of DNA. In this method no carrier DNA is papers, required and stable transformants are produced. This method works with mouse fibroblast and chick embryo. LIPOSOME MEDIATED GENE TRANSFER. Liposomes are spheres of lipids which can be used to transport molecules into the cells. These are artificial vesicles that can act as delivery agents for exogenous materials including transgenes. They are considered as spheres of lipid bilayers surrounding the molecule to dance therapy, be transported and promote transport after fusing with the cell membrane.
Liposomes for statement, use as gene transfer vehicles are prepared by adding an appropriate mix of therapy research paper, bilayer constituents to an aqueous solution of DNA molecules. In this aqueous environment, phospholipid hydrophilic heads associate with water while hydrophobic tails self-associate to exclude water from within the lipid bilayer. This self-organizing process creates discrete spheres of continuous lipid bilayer membrane enveloping a small quantity of DNA solution. The liposomes are then ready to be added to education papers, target cells. Germline transgenesis is possible with liposome mediated gene transfer.Lipofection is the most common and generally utilized gene transfer technique in the recent years and it utilizes cationic lipids. The combined DNA and cationic lipids act instantaneously to form structures called as lipoplexes that are more complex in structure than the simple liposomes. Therapy Paper! Cationic lipids have a positive charge and these form cationic liposomes which interact with the negatively charged cell membrane more readily than uncharged liposomes.
This leads to a fusion between cationic liposome and essay lifestyle prevent the cell surface resulting in quick entry by endocytosis and the delivery of the DNA directly across the plasma membrane. Cationic liposomes can be produced from a number of cationic lipids that are commercially available and sold as an in vitro-transfecting agent, termed lipofectin. However this pathway would usually result in the fusion of lipoplexes with lysosomes and undergo degradation. This problem is overcome by utilizing the neutral helper lipids, which are generally included along with the cationic lipid. This allows entrapped DNA to escape the endosomes, reach the nucleus and get access to the cell’s transcriptional machinery.The endosomal structure is destroyed by increasing the osmotic pressure created by the lipids’ buffering action within the endosomes and by the fusion of the lipid with the dance paper endosomal membrane. The ability of a lipid to destroy endosomes is one of the main characteristics of a transfection reagent. 2. Long term stability. 4. Protection of nucleic acid from thesis, degradation.
5. particularly proficient at delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 6. Dance! cationic lipids effectively transfect suspension cells. 1) Cannot deliver genes to terminally differentiated cells. A rapid and simple technique for healthy prevent diabetes, introducing genes into a wide variety of microbial, plant and animal cells, including E. coli, is electroporation. Research! Electroporation uses electrical pulse to thesis, produce transient pores in the plasma membrane thereby allowing macromolecules into dance therapy, the cells. These pores are known as electropores which allow the molecules, ions and water to pass from one side of the essays membrane to another. The electropores reseal spontaneously and dance research paper the cell can recover. The pores can be recovered only if a suitable electric pulse is applied .The formation of electropores depends upon bibliography the cells that are used and the amplitude and duration of the therapy research electric pulse that is applied to them. Essay Lifestyle Prevent! When subjected to electric shock, cells take up exogenous DNA from the suspending solution.
Once inside the cell, the DNA is therapy research, integrated and the foreign gene will express. A proportion of these cells become stably transformed and can be selected if a suitable marker gene is carried on the transforming DNA. The most critical parameters are the intensity and duration of the bibliography electric pulse, and these must be determined empirically for different cell types. Electric currents can lead to dramatic heating of the cells that can results in cell death. Heating effects are minimized by using relatively high amplitude, a short duration pulse or by using two very short duration pulses. However, once optimal electroporation parameters have been established, the method is simple to carry out and highly reproducible. Many different factors affect the dance research efficiency of electroporation, including temperature, various electric-field parameters (voltage, resistance and capacitance), topological form of the DNA, and various host-cell factors (genetic background, growth conditions) and personal layout post-pulse treatment. General applications of electroporation. 1. Dance Therapy! Introduction of exogeneous DNA into animal cell lines, plant protoplast, yeast protoplast and bacterial protoplast.
2. Electroporation can be used to essays by burgmen, increase efficiency of dance research paper, transformation or transfection of bacterial cells. 3. Wheat, rice, maize, tobacco have been stably transformed with frequency upto 1% by this method. 4. Genes encoding selectable marker may be used to papers, introduce genes using electroporation. 5. To study the transient expression of molecular constructs. 6. Electroporation of therapy paper, early embryo may result in the production of transgenic animals. 7. Hepatocytes, epidermal cells, haematopoietic stem cells, fibroblast, mouse T and B lymphocytes can be transformed by this technique. 8. Naked DNA may be used for gene therapy by applying electroporation device on essay healthy, animal cells. Advantages of electroporation. 1. Therapy Research! Method is fast. 3. Applied for a number of cell types.
4. Simultaneously a large number of cell can be treated. 5. High percentage of stable transformants can be produced. 1)Limited effective range of. 1 cm between the electrodes. 2)Surgical procedure is required to place the electrodes deep into the internal organs. 3)High voltage applied to tissues can result in irreversible tissue damage as a result of thermal heating. The technique has high input costs, because a specialized capacitor discharge machine is required that can accurately control pulse length and amplitude. 4) Larger numbers of ucl mdres, cells may be required than for other methods because, in many cases, the most efficient electroporation occurs when there is up to 50% cell death. Sonication is the act of applying sound energy to agitate particles in a sample. Ultrasonic frequencies (20 kHz) are usually used, leading to the process also being known as ultrasonication . Sonoporation, or cellular sonication, is the use of dance therapy, these ultrasonic frequencies for modifying the permeability of the cell plasma membrane.
This technique is statement, usually used in molecular biology and therapy research non-viral gene therapy in order to allow uptake of large molecules such as DNA into education papers, the cell, in dance research paper a cell disruption process called transfection or transformation. Sonoporation employs the acoustic cavitation of microbubbles to enhance delivery of such large DNA molecules. Although the early results could achieve only 10- to 30-fold increases in transfection efficiency in vitro, technical refinements have lead to research, enhancements of up to several thousand fold in vitro ,sufficient to encourage ultrasonication mediated gene transfer in vivo. 1) It is non-invasive and dance well tolerated, 2)extraordinary safety record over a wide range of frequency and intensity. 3)high levels of public acceptability and understanding. there are highly sophisticated, flexible, cost-effective and readily available diagnostic and therapeutic systems that can achieve site-specific transfer of ultrasound energy almost anywhere in the body, except perhaps the lung. 4) The bioactivity of this technique is similar to, and in for unemployment some cases found superior to, electroporation. 5) in vivo is relatively nontoxic.
1)Extended exposure to low-frequency (MHz) ultrasound has been demonstrated to result in complete cellular death (rupturing) as well as microvascular hemorrhage and research paper disruption of tissue structure, thus cellular viability must also be accounted for when employing this technique. 2) Whether ultrasound affects later steps in the transfection process, particularly plasmid entry into the nucleus, remains unclear. Sonoporation is under active study for thesis, the introduction of foreign genes in tissue culture cells, especially mammalian cells. Sonoporation is also being studied for therapy research paper, use in targetedGene therapy in vivo, in for unemployment a medical treatment scenario whereby a patient is given modified DNA, and an ultrasonic transducer might target this modified DNA into specific regions of the patient’s body. In microinjection DNA can be introduced into cells or protoplast with the dance help of very fine needles or. glass micropipettes having the diameter of 0.5 to 10 micrometer. The DNA may be directly delivered into the nucleus or in the cytoplasm. Some of the DNA injected may be taken up by the nucleus.
The desired gene in the form of plasmid or alone is injected directly into the plant protoplast. Injection into the nucleus is more efficient than that into the cytoplasm. Essays! Micro-injection is carried out with automatic equipments (robotics) using a micro-needle. Computerized control of holding pipette, needle, microscope stage and video technology has improved the efficiency of this technique. Advantages of dance therapy research, microinjection. 1. Frequency of stable integration of DNA is far better as compare to other methods. 2. Method is effective in transforming primary cells as well as cells in established cultures. 3. The DNA injected in this process is subjected to less extensive modifications. 4. Mere precise integration of healthy lifestyle prevent diabetes, recombinant gene in limited copy number can be obtained. 2. Skilled personal required.
3.Has not been successful for many plant cells . 4. Embryonic cells preferred for research, manipulation. 5. Thesis Statement For Unemployment! Knowledge of therapy research paper, mating timing, oocyte recovery is essential. 6. Method is useful for protoplasts and not for statement, the walled cells. 7. Process causes random integration. 8. Dance Research! Rearrangement or deletion of host DNA adjacent to site of thesis statement, integration are common. 9. The technique is dance therapy, laborious, technically difficult, and limited to the number of cells actually injected. Applications of microinjection. 1. Process is applicable for essays by burgmen, plant cell as well as animal cell but more common for animal cells. 2. Technique is ideally useful for producing transgenic animal quickly. 3. Procedure is important for gene transfer to embryonic cells.
4. Applied to inject DNA into dance research paper, plant nuclei. 5. Healthy Lifestyle Prevent Diabetes! Method has been successfully used with cells and protoplast of. tobacco, alfalfa etc. Fig: microinjection in mouse egg. Biolistics or particle bombardment is a physical method that uses accelerated microprojectiles to. deliver DNA or other molecules into intact tissues and cells. Biolistics transformation is therapy research paper, relatively new and novel method amongst the physical methods for artificial transfer of exogenous DNA. The nucleic acid is delivered through membrane penetration at a high velocity, usually connected to microprojectiles like microscopic particles of ucl mdres, tungsten or gold coated into cells using an electrostatic pulse, air pressure, or gunpowder percussion. As the particles pass through the cell, the DNA becomes free to integrate into the plant-cell genome.
The gene gun is a device that literally fires DNA into therapy research paper, target cells . The DNA to be transformed. into the cells is coated onto microscopic beads made of either gold or tungsten. Beads are carefully. coated with DNA. The coated beads are then attached to the end of the for unemployment plastic bullet and loaded into. the firing chamber of the therapy paper gene gun. An explosive force fires the bullet down the essays barrel of the dance therapy research gun. towards the target cells that lie just beyond the end of the education research barrel.
When the bullet reaches the end of. the barrel it is caught and stopped, but the dance therapy paper DNA coated beads continue on toward the target cells. Some of the beads pass through the cell wall into bibliography, the cytoplasm of the target cells. Here the bead and. the DNA dissociate and dance paper the cells become transformed. Once inside the target cells, the DNA is. solubilised and may be expressed. 1. Biolistics technique has been used successfully to transform soyabean, cotton, spruce, sugarcane, papaya, corn, sunflower, rice, maize, wheat, 2. Genomes of subcellular organelles have been accessible to genetic. manipulation by ucl mdres, biolistic method. 3. Dance Therapy Research Paper! Mitochondria of plants and chloroplast of chlamydomonas have been.
4. Method can be applied to filamentous fungi and yeast (mitochondria). 5. The particle gun has also been used with pollen, early stage. embryoids, meristems somatic embryos, plant cells, root section, seeds and pollen. 6. This approach has been shown to sources, be effective for transferring transgenes into mammalian cells in vivo . 1. Requirement of protoplast can be avoided. Unlike electroporation and dance paper microinjection, this technique does not require protoplasts or even single-cell isolations and is applicable to even intact plant tissue.
2. Walled intact cells can be penetrated. 3. Manipulation of for unemployment, genome of subcellular organelles can be achieved. 4. This is also a highly mechanized or robotic mediated technique in dance therapy research paper which the. speed of the micro-projectile particles is controlled by ucl mdres thesis, foolproof mechanisms. 5) It is also gaining in use as a method for transferring DNA construct into whole animals.
6) This technique is fast, simple and safe, and it can transfer genes to a wide variety of tissues. 7)there appears to be no limits to the size or number of genes that can be delivered. 1. Integration is random. 2. Therapy Research Paper! Requirement of equipments. 3.It can be a challenge to for unemployment, obtain a sufficient number of cells modified by this method to see a biologically significant effect. A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions.
At the dance therapy research paper site of the beam impact, due probably to local temperature changes, the cell membrane modifies its permeability. As a consequence of the hit, circular areas, whose radius may be apparently regulated by changing the sources bibliography irradiation time and/or the radiation intensity (energy), appear on dance therapy paper, the wall, last for a short time and fade spontaneously . It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to slip into the cell. It is well established that a strong pressure wave, known as a laser-induced stress wave (LISW), accompanies laser-induced plasma. We have extended their method to deliver macromolecules, such as genes. Plasmid DNA (circular DNA residing in bacterial cytoplasm) is used as a vector of the gene of interest. We inject the plasmid into target tissue, on which a laser target is placed, and education papers research subject the target to irradiation with a high-intensity, nanosecond laser pulse to induce plasma and hence an LISW.
By placing optically transparent material on the target, the plasma is therapy paper, confined, resulting in an increase in the LISW’s impulse. Interaction of essays, tissue cells with the LISW allows plasmid to enter the cytoplasm. 1) it only takes advantage of the presence of phenol-red, a normal cell medium component, with no need of addition of extraneous substances; (2) it is a very mild treatment virtually suitable for any cell type and. (3) it allows transfection of selected cells even in the presence of cells of different type (providing that they are morphologically distinguishable). 4) . it is rapidly replacing virus mediated techniques as they have serious side effects caused by research paper, immune response and limited targeting characteristics rendering them inappropriate for clinical application. 5) The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude. 6) . It enables minimally invasive tissue interaction. Thus there are a number of ways by which the genes can be introduced into the cells. With the advent of molecular tools and technologies it is now comparatively easy to personal layout, introduce gene into cells without losing its integrity and biological activity.
Moreover the therapy paper recent development in molecular biology has made the transfer of gene with great accuracy to for unemployment, the target cell. The transfer of gene through different gene transfer technologies has cured a number of diseases. Research is on progress to cure those diseases which cannot be cured by using drugs. Moreover the treatment of diseases by gene transfer provides better result for a prolong period of time. It is the need of hour to discover new and dance therapy research paper cheap method of gene transfer technologies so to make the treatment of the diseases a little easier and healthy affordable.
Improvements in dance research paper gene transfer are required in terms of transfection approaches to allow improved transgene uptake efficiencies. 1)Society of Applied Sciences. Gene Transfer Technologies and their Applications: K.H.KHAN. Medical Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India. 2)Principles of gene manipulation: R.W.Old ,S.B. By Burgmen! Primrose and R.M.Twymann ‘ sixth edition,chapter 10 and therapy 11. 3) BIOTECHNOLOGY 2020-From the Transparent Cell to. the Custom-Designed Process–Prof.
Gerhard Kreysa Dr.-Ing. E.h. Personal Layout Uk! Dr.h.c. Dr. R??diger Marquardt. 4) INTRODUCTION TOBIOTECHNOLOGY AND GENETIC ENGINEERING.
5)From genes to dance paper, genomes- concepts and applications of statement for unemployment, DNA technology–Jeremy W Dale, Malcom von Schantz. Search our thousands of essays: If this essay isn't quite what you're looking for, why not order your own custom Science essay, dissertation or piece of dance therapy paper, coursework that answers your exact question? There are UK writers just like me on hand, waiting to help you. Each of us is qualified to a high level in our area of expertise, and we can write you a fully researched, fully referenced complete original answer to your essay question. Just complete our simple order form and sources bibliography you could have your customised Science work in your email box, in as little as 3 hours. This Science essay was submitted to us by a student in order to help you with your studies. This page has approximately words. If you use part of this page in your own work, you need to provide a citation, as follows: Essay UK, Essay: Gene transfer techniques – pros and cons . Dance! Available from: http://www.essay.uk.com/essays/science/essay-gene-transfer-techniques-pros-and-cons/ [04-10-17]. If you are the personal layout original author of this content and no longer wish to have it published on our website then please click on dance research, the link below to request removal:
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4 Steps to therapy paper Writing a Job-Winning Resume. Writing your resume, or even updating your resume, can be a scary, daunting task. But with the sources bibliography, right approach and know-how, you can create your own standout document in no time. Therapy Paper! And the good news is we’re here to help. Healthy Lifestyle Diabetes! We’ve broken down the resume-writing process into 4 simple steps to dance therapy research paper make it easier for you to get started!
Our Resume Writing Guide gives you an overview of statement each section of your resume, followed by some quick tips and therapy actual resume examples that you can use as model for your own resume. Essay Healthy Lifestyle Prevent! Every great resume begins with a strong summary statement. This is a short description of who you are professionally that includes a brief list of your top skills. Your summary statement is the first thing that recruiters will see. Therapy Paper! So think of thesis it as your big chance to catch someone’s attention. Dance Therapy Research! You also want to show hiring leaders that you’re a great fit for sources the job. You can do this by using the skills and dance research paper keywords that are in the job description in your summary statement – just make sure you only use those that reflect your experience (no exaggerating or lying on your resume!). Your statement can be written in either sentence form or bullet-point form and should be short, but effective – no more than 3 sentences or bullet points; it should also contain the following information: Your professional title Top 2-3 skills Specific expertise, professional traits, accomplishments. Need help writing your own professional summary statement? Our Resume Builder has hundreds of expertly written summary statements and career-specific phrases that you can easily and quickly add to your resume.
Registered nurse with 9 years of experience in medical centers and operating rooms ICU and ER experience in 40+ bed units Strong organizational and interpersonal skills. Dedicated assembly line worker with 5 years of education papers research experience. Well-versed in therapy research paper machinery assembly and production line efficiency . Fast learner who picks up new processes and technologies easily. Senior Sales Associate with excellent customer relations skills in business-to-business sales Increased global sales by essays by burgmen, 20% over therapy research paper, the last two years, exceeding goals each quarter for the past year Seeking new sales challenge with management opportunities. Statement! Experienced veterinary technician with 3 years of dance therapy experience in delivering comprehensive care to animals at animal rescue facilities and ucl mdres shelters. Seeking to use shelter experience and formal training at research, a respected veterinary office. Statement For Unemployment! Responsible cashier experienced at managing front-of-store needs in busy environments. Friendly and energetic with strong communication and organizational abilities.
Seeking role of dance research paper increased responsibility where strengths in service and for unemployment sales will be valuable. Motivated sales professional with 8 years of fashion retail sales experience who truly enjoys helping customers find their best fit and style. Dance Therapy! Highly results-oriented and essay lifestyle prevent diabetes energetic, with unsurpassed customer relations skills. The skills section – often called the #8220;Qualifications#8221; or #8220;Areas of dance research Expertise#8221; section of healthy lifestyle your resume is a list of your top skills. This section of dance your resume is your chance to showcase your abilities to employers in a quick and research easy-to-read way. Although this section is typically short and concise (think two columns of dance 3 to healthy lifestyle diabetes 4 bullet points), it contains very important information about your ability to perform a particular type of dance paper job.
Hiring managers should be able to scan your resume and find this list of skills easily. Here are a few helpful tips for writing a skills section that will get you noticed. Target your skills to the job : This is essays, one of the best ways to customize your resume for the job you are applying to. Read the job description and dance research paper list all of the required and desired skills for the position. Then, see if you have any skills that match up with those on your list; these are the abilities you should include in your skills section. This way, when hiring managers skim your resume, they will see that you have the ucl mdres thesis, skills they’re looking for in a candidate. Include transferable skills : Transferable skills are skills that cross from one career field to therapy research paper another. These skills can be applied to a variety of positions.
Some examples include communication skills, presentation or public speaking skills, any foreign language skills you have, social media skills, organizational and planning skills, and management and leaderships skills. Use keywords : If you are applying to a job online and are asked to upload your resume or fill in an application online, chances are you are entering your information into an application tracking system. Essays By Burgmen! This is a machine that companies use to scan a resume for keywords specific to the job. Be sure to pick out keywords from the therapy research paper, job description and papers your industry and use them in dance therapy your resume and application to make sure it gets past this machine. Using keywords directly from the employer will increase your chances of getting noticed by the hiring manager. Need help writing a skills section that gets you noticed? Our Resume Builder has hundreds of career-specific phrases that you can easily and quickly add to your own resume. While all parts of your resume are equally important, your work history section is where you will be discussing your direct experience and your accomplishments, and essay diabetes it’s critical to dance therapy research get this one right.Let’s start with the basics. Your work history section should include the following: Positions and/or titles you held Names of organizations where you were employed City and state of each organization Employment periods for each job, written as Month/Date – Month/Date Brief descriptions of your experience in bullet format. This section will be a combination of your job responsibilities and duties along with your work achievements. Hiring managers will be looking for your achievements and successes, not just your responsibilities, so it’s important to include both.
When listing your responsibilities and personal uk duties, be sure to use action verbs to increase the strength of your writing and make potential employers take notice. Some action verbs to use include: Communicated Negotiated Joined Reported Customized Initiated Planned Budgeted Developed Prepared Contributed Ensured Provided Led. It takes practice and time to dance therapy paper come up with your accomplishments and successes and to add numbers to them numbers such as how much money you saved the company, how you increased efficiency, or how many sales you achieved in a certain period of time. However, using two or three bullets under each job to describe your successes, along with metrics, will land you an thesis for unemployment, interview. Need help writing a work experience section that shows your value? Our Resume Builder has hundreds of career-specific phrases that you can easily and quickly add to your own resume. Dance Research Paper! Bayle Industries, Hutchins Creek, MD. July 2010 – present. Prioritized and sources bibliography managed multiple administrative projects while managing day-to- day tasks such as receiving and distributing mail, maintaining conference rooms, managing calendars, and maintaining office supplies. Increased conference room scheduling efficiency by 25% by implementing new company-wide online booking system.
Provided travel arrangement support for 25+ employees per dance therapy, month for offsite meetings and national conferences. Managed operations and logistics for annual onsite company conference for 150 employees and papers 200+ guest attendees. Therapy Research! Parkview Elementary School, Summerfields, PA. September 2014 – May 2016. Assisted in a classroom of 25 elementary school children, ages 5-6, for two consecutive school years. Ucl Mdres! Engaged children with diverse and enjoyable activities involving theatrical play, musical instruments and hands-on learning. Offered detailed weekly reports that outlined each child’s activities and implemented new email letter system to offer reports online, saving the faculty 4 hours per week. Ft.
Hood, Killeen, TX. January 2008 – August 2015. Developed and led 5 new training programs over 12 months in preparation for dance research combat. Performed reconnaissance operations in compromised regions to develop combat training programs. Performed as a fire team member during situational training exercises and all infantry dismounted battle drills. Communicated urgent orders and directions effectively to team of 200+ military personnel.
Your educational background can be an important part of your resume and should communicate your background quickly and simply to potential employers. Even if you don’t have previous experience in a particular job, you may increase your chances of being considered for the position if you can show that you have taken courses that are relevant to the position at hand. Education Research! Here are some tips to help make your education section work for you: List the highest level of education first. This will help catch the hiring manager’s eye and ensure that he or she is able to determine your education level quickly. If you are recent graduate without a ton of work experience, then you can place your education section at the top of therapy paper your resume for emphasis. Include your educational information in this order: Degree or diploma name is thesis statement, listed first.
Major(s) follow the dance paper, degree. Statement! If you have minors, these are optional to list but it’s recommended to dance therapy research list them if they are relevant to uk the job you are applying for. List the date you received the degree or diploma by year (for example, 2003). Dance Therapy Research! If you are soon-to-be grad, you can list your expected graduation date. Employment periods for each job, written as Month/Date – Month/Date Do not include your GPA on your resume unless you are a recent graduate. If you don’t have a degree, never fear! If you have an bibliography, associate’s degree, you can list it in the same way as above. Dance Therapy Paper! It is also perfectly acceptable to list any college experience you do have, even if you didn’t graduate. Thesis! In the dance research, absence of a college degree, it’s extremely important to list any professional training or certificates you have received especially if they are relevant to the job you are applying for. Then, make sure to emphasize your work experience section as much as possible to show employers that you are qualified for the position, whether or not you have a degree.
Need help writing an for unemployment, education section that shows your value? Our Resume Builder can help you quickly and effectively outline your classroom achievements. More Articles about How to Write Your First Resume. 3 Critical Mistakes to dance therapy paper Avoid on Your First Ever Resume. Tips On Creating A Resume For A New Career. How to lifestyle Write a Resume When You Have No Work Experience. Get Hired In 30 Seconds: Resume Summary Tips.
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Essay On Mountain Trip Essays and Research Papers. My Riding Trip in the Mountains One day in April I was playing games in my room and my dad asks me if I want to go to dance research, . the mountains to ride four wheelers and dirt bikes, and I said yes. So me and personal my dad walked up to dance paper, the garage and put the trailer on the truck. The next day was Thursday and I had to load everything on to the trailer and into the truck. The first thing I had to load up was the clothes, sheets, blankets, tooth brushes, tooth paste, and a couple towels. Essays? Then I had to load up. English-language films 1019 Words | 3 Pages. TRIP Essay Conflict Resolution Who Do We Hire? Participants: John, Jim (the Director), Laura, Karl, Keith and . Therapy? Celeste Setting: Mental health center Situation: An opening for a full-time therapist has been created by one of the staff therapists quitting.
Questions 1. Name at least one topic (T) issue, one relational (R) issue, one identity (I) issue, and one process (P) issue of this conflict. T Issue = The most obvious topic issue during this conflict is the need to fill the position. 1199 Words | 3 Pages. Mount Kailas is also great in ucl mdres religious value. It is the holy mountain to therapy paper, four different religions: Hinduism, Jainism, Buddhism, and Bon.
There . is a lot of sources border trafficking for every year thousands of pilgrims from all the four different religions go to the holy mountain to complete the great pilgrimage, the Kora from all around Asia. This is the journey that Colin Thubron sets out to complete. At the beginning of the book To a Mountain in Tibet (2011) Colin Thubron writes, “Im doing this on account. Death , Himalayas , Indus River 985 Words | 3 Pages. Across a hundred Mountains In Across a Hundred Mountains Adelina started her journey to the other side. She had gone to therapy paper, the . train station where she sold quesadillas with Dona Martina.
When she sat down she noticed that in front of her there was a man and a young girl talking about leaving to the other side. The girl asked her dad what did they need to thesis statement, do in order to get there, thats when Juana heard that you needed to catch a train to Tijuana then find a good coyote to take you to therapy research paper, the other side. Aerosmith , Mother , Need 1078 Words | 3 Pages. Main Characters and their Connections to Nature Charles Frazier’s novel Cold Mountain takes place during the prevent, time of the civil war. Paper? However, . instead mainly focuses on each character and the life lessons they learn throughout the ucl mdres thesis, story. As the therapy paper, novel progresses we the characters go through different arduous journeys. The novel Cold Mountain is made up of two separate journeys occurring at statement for unemployment the same time that eventually come together at the end.
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We gathered around 8 o’clock in the morning at the Tanjung Bungah. By Burgmen? We were lucky because the weather was fine and sunny. The golden rays of the paper, early morning sun brought much warmth and cheer. Sources Bibliography? My friends and I was so excited and thrilled to dance paper, wait for the trip to start. Before we start our journey, En.Hamdan, who is the trip organizer’s, gave us a. Batu Ferringhi , Cities, towns and ucl mdres villages in Penang , George Town, Penang 1041 Words | 3 Pages. The Trip Hello, my name is Harry Bailey and dance therapy I have recently went on a trip to Canterbury with 29 very unique pilgrims. The . trip was interesting and exciting to say the by burgmen, least.
I definitely enjoyed meeting and interacting with these pilgrims and having the opportunity The weather could not decide between sunny or rainy on most days, but no matter what it was it was always hot. Therapy Paper? Some of the pilgrims came on the trip with impairments, but got the chance to get them fixed. Thesis? The cook was able to therapy, get his. Elaine Benes , George Costanza , Jerry Seinfeld 934 Words | 3 Pages. Our trip 10 DAYS TRIP 16/5/2013 TO 26/8/2013 8 DAYS Flight 12pm noon meet. Education Papers? Take taxi meet up. Dance Therapy Paper? Arrive before 1pm.Check in personal statement layout . have some food snack hehe. 4pm to therapy research paper, 10pm (flight to china) -gt;10pm to bibliography, 8am (flight to therapy paper, jeju from china) Get ready before 6am to check in board. Personal Statement Layout Uk? 17Th August 2013 Saturday12:30pm -1pm(DAY 1 )Main schedule:Wifi eggmapTrick art museumSeafood - try seafood porridge , abalone , anyting that is seafood. MUAHAHAHHAloveland | Upon arrival to dance paper, Jeju around .Task:Egg wifiRequest map. Administrative divisions of South Korea , Busan , Jeju Air 587 Words | 3 Pages.
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Yet we started this trip along with my. Forest , Hotel , Reach 1627 Words | 5 Pages. Saving the Mountains Christina Samples Kaplan University Final Project College Composition II Professor Mark Kusnir . Table of Contents: I. Essay II. References III. Formal Letter IV.
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